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Your StackDNA Report

Sarah M.
March 23, 2026
23andMe raw file
47 / 62 detected
Overview

At a Glance

5
Elevated-risk variants
12
Moderate-impact variants
30
Within normal range

Your strongest signals are in methylation and neurotransmitter balance. A compound heterozygous MTHFR pattern (C677T + A1298C) reduces folate enzyme activity by roughly 50-60%, which combined with your slow COMT genotype means methylation support needs to start low and build gradually.

Your detoxification and inflammation pathways also show meaningful variants. GST deletions reduce glutathione conjugation capacity, and your IL-6 promoter variant is associated with a more active inflammatory baseline. Both are addressable with targeted support.

Your Input

Current Medications

How each medication you reported interacts with this protocol.

! Levothyroxine 75 mcg Monitor
Your thyroid medication absorbs best on an empty stomach. Take all supplements at least 4 hours apart from levothyroxine to avoid interference with absorption.
Timing: Take levothyroxine first thing in the morning. Move all morning supplements to breakfast or later.
✓ Escitalopram 10 mg No conflict
No direct conflicts with your recommended supplements. 5-HTP, tryptophan, and SAMe have been excluded from your protocol as a precaution with SSRI use.
Your Input

Current Supplements

Whether to keep, adjust, replace, or stop each supplement you're already taking.

→ Folic acid 400 mcg Replace
Your MTHFR compound heterozygous pattern means you convert folic acid poorly. Switch to methylfolate (5-MTHF), which bypasses the impaired enzyme entirely.
Replace with: L-Methylfolate (5-MTHF) 400 mcg
↻ Magnesium citrate 200 mg Adjust
Magnesium is well-indicated for your genotype, but glycinate form is better tolerated and more effective for your sleep and neurotransmitter goals. Consider increasing to 300 mg.
✓ Vitamin D3 2000 IU Keep
Good fit for your VDR variant. Your recent lab showed 28 ng/mL, which is below optimal. Consider increasing to 4000 IU and adding K2 (MK-7) for calcium routing.
× B-complex (standard) Stop
Most standard B-complexes contain folic acid and cyanocobalamin, which are poorly utilized with your MTHFR and slow COMT variants. Your protocol includes the specific B vitamins you need in their correct forms.
Your Protocol

What to Take

Core — genetically essential
L-Methylfolate (5-MTHF)
Calcium salt
400 mcg AM Morning
MTHFR C677T MTHFR A1298C
Bypasses your reduced MTHFR enzyme. Starting dose is conservative due to slow COMT.
Riboflavin (Vitamin B2)
Riboflavin 5'-phosphate
25 mg AM Morning
MTHFR C677T
MTHFR uses FAD (from B2) as its cofactor. Riboflavin can partially rescue reduced enzyme activity.
Magnesium Glycinate
300 mg PM Evening
COMT MTHFR GAD1
Supports COMT function, nervous system balance, and sleep. Glycinate form preferred for absorption and calming effect.
Omega-3 (EPA+DHA)
Triglyceride form fish oil
1500 mg With food
FADS1 IL-6 APOE
Your FADS1 variant reduces EPA/DHA conversion. Preformed omega-3 bypasses this bottleneck and addresses inflammatory tone.
Targeted — pathway support
N-Acetylcysteine (NAC)
600 mg AM Morning
GSTM1 GSTT1
GST deletions reduce glutathione conjugation. NAC is the rate-limiting precursor for glutathione synthesis.
Vitamin D3 + K2 (MK-7)
4000 IU + 100 mcg With food
VDR Taq VDR Bsm
VDR variants reduce receptor sensitivity. Higher D3 intake compensates. K2 ensures proper calcium routing.
Curcumin Phytosome
500 mg With food
IL-6 TNF-α
Targets your elevated inflammatory signaling baseline. Phytosome form has 29x better absorption than standard curcumin.
Supportive — quality of life
L-Theanine
200 mg PM Evening
COMT GAD1
Supports GABA activity and calms catecholamine excess from slow COMT without sedation.
CoQ10 (Ubiquinol)
100 mg With food
NQO1
Your NQO1 variant impairs CoQ10 recycling. Ubiquinol is the reduced, bioavailable form.
Protocol Rationale

Why These Doses

Critical

Slow COMT + MTHFR Compound Het — Methylation Bottleneck

COMT rs4680 A/A · MTHFR rs1801133 C/T · MTHFR rs1801131 G/T

Your slow COMT clears methyl groups slowly, while your compound heterozygous MTHFR reduces methylfolate production. Starting methylfolate at full dose would likely cause irritability, anxiety, and insomnia. Your protocol begins with cofactors only (magnesium, B2, omega-3) and introduces methylfolate at 400 mcg in Phase 3 after your system is supported.

High

GST Deletions + IL-6 Promoter — Oxidative Stress Amplification

GSTM1 del/del · GSTT1 del/del · IL-6 rs1800795 G/G

Dual GST deletions significantly reduce Phase II detoxification capacity, while your IL-6 variant drives a more active inflammatory baseline. Together, this creates a feedback loop where inadequate glutathione recycling amplifies inflammatory damage. NAC at 600 mg and curcumin phytosome are prioritized to break this cycle.

High

VDR Variants + Low Vitamin D Lab — Receptor Resistance

VDR rs731236 A/A · VDR rs1544410 C/T

Your VDR polymorphisms reduce vitamin D receptor sensitivity, which means your body needs a higher circulating level to achieve the same effect. Your recent lab (28 ng/mL) is below the 40-60 ng/mL target for your genotype. Dose is set at 4000 IU rather than the standard 1000-2000 IU, with K2 to handle increased calcium mobilization.

Contraindications

What to Avoid

×

Folic acid (synthetic)

Your MTHFR compound heterozygous pattern cannot efficiently convert folic acid to its active form. Unmetabolized folic acid may accumulate and compete with methylfolate for receptor binding.

×

5-HTP, Tryptophan, and SAMe

Contraindicated with your SSRI (escitalopram). These compounds affect serotonin pathways and risk serotonin syndrome when combined with SSRIs.

!

High-dose methyl donors

With slow COMT, aggressive methylation support (high-dose methylfolate, TMG, SAMe, methyl-B12 above 1000 mcg) can cause anxiety, insomnia, and irritability. Titrate slowly.

!

EGCG (green tea extract)

EGCG inhibits COMT activity. With your already-slow COMT, concentrated green tea extract would further impair catecholamine clearance. Moderate green tea drinking is fine.

Getting Started

How to Start

Foundation Weeks 1–2
Cofactors and anti-inflammatory support first. No methylation donors yet — your slow COMT needs a stable foundation before adding methyl groups.
Magnesium glycinate300 mg evening
Riboflavin (B2)25 mg morning
Omega-3 (EPA+DHA)1500 mg with food
Vitamin D3 + K24000 IU + 100 mcg
Build Weeks 3–4
Add detox and antioxidant support. Your glutathione pathways need time to upregulate before adding methylation load.
NAC600 mg morning
Curcumin phytosome500 mg with food
CoQ10 (ubiquinol)100 mg with food
L-Theanine200 mg evening
Methylation Weeks 5–6
Introduce methylfolate last, at the lowest effective dose. If you feel wired or anxious, hold the dose for a week before increasing.
L-Methylfolate (5-MTHF)400 mcg morning
Genetic Detail

Variant-by-Variant

MTHFR
rs1801133
C / T
C677T heterozygous. Approximately 35% reduced enzyme activity. Impairs folate-to-5-MTHF conversion and can raise homocysteine.
A
MTHFR
rs1801131
G / T
A1298C heterozygous. Less impactful alone, but compound heterozygosity with C677T is functionally similar to C677T homozygous.
A
COMT
rs4680
A / A
Val158Met homozygous (Met/Met). Slow COMT. Reduced catecholamine clearance. More sensitive to methyl donors, stress, and stimulants.
A
MTRR
rs1801394
A / G
Heterozygous. Modestly reduced methionine synthase reductase activity. Supports the case for methylcobalamin over cyanocobalamin.
B
MTR
rs1805087
A / A
Wild-type. Normal methionine synthase function. No additional B12 support needed beyond standard methylation protocol.
A
GSTM1
deletion
del / del
Homozygous deletion. No GSTM1 enzyme produced. Reduces Phase II glutathione conjugation of carcinogens and oxidative byproducts.
A
GSTT1
deletion
del / del
Homozygous deletion. Combined with GSTM1 deletion, significantly impairs overall glutathione-dependent detoxification capacity.
A
NQO1
rs1800566
C / T
Heterozygous. Reduced CoQ10 recycling efficiency. Ubiquinol form preferred over ubiquinone for supplementation.
B
IL-6
rs1800795
G / G
Homozygous high-expression allele. Associated with higher baseline IL-6 production and more active inflammatory signaling.
B
TNF-α
rs1800629
G / G
Wild-type. Normal TNF-α promoter activity. Inflammatory risk is primarily driven by IL-6 in your case.
B
VDR
rs731236
A / A
TaqI homozygous variant. Reduced vitamin D receptor expression. Higher circulating 25-OH-D needed to achieve normal receptor activation.
A
VDR
rs1544410
C / T
BsmI heterozygous. Compounds with TaqI to further reduce vitamin D receptor sensitivity. Target 40-60 ng/mL for your genotype.
A
FADS1
rs174546
C / T
Heterozygous. Reduced delta-5 desaturase activity. Impairs conversion of ALA to EPA/DHA, making preformed omega-3 supplementation more important.
A
Validation

Recommended Labs

Homocysteine
Confirms whether your MTHFR variants are causing functional methylation stress. Target < 8 μmol/L.
Essential
25-OH Vitamin D
Recheck in 8 weeks at new dose. Target 40-60 ng/mL given your VDR variants.
Essential
hs-CRP
Baseline inflammatory marker. Tracks whether curcumin and omega-3 are reducing your IL-6-driven inflammation.
Essential
Methylmalonic Acid (MMA)
Confirms B12 adequacy at the tissue level. Useful given your MTRR variant and switch to methylcobalamin.
Recommended
RBC Magnesium
Serum magnesium is unreliable. RBC level confirms whether your 300 mg dose is sufficient for COMT and sleep support.
Recommended
GGT
Adjunct liver marker. Relevant given your dual GST deletions and reduced detox capacity.
Optional

Important Disclaimer

This is a sample report with fictional data for illustration purposes. Real reports are generated from your actual genetic data and health questionnaire responses.

StackDNA reports are for educational and informational purposes only and do not constitute medical advice, diagnosis, or treatment. Consult a qualified healthcare provider before starting any supplement regimen.

StackDNA
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